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Human Phospho-RB (T826) and Total RB PharmaGenie ELISA Kit (SBRS1946)

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SKU:
SBRS1946
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
  • Human Phospho-RB T826 and Total RB PharmaGenie ELISA Kit SBRS1946
  • Human Phospho-RB T826 and Total RB PharmaGenie ELISA Kit SBRS1946
€1,469

Description

Human Phospho-RB (T826) and Total RB PharmaGenie ELISA Kit

Product SKU:SBRS1946
Size:96T
Application:This ELISA kit recognizes Human RB phosphorylated at site Threonine-826 as well as total RB.
Uniprot:P06400
Gene ID:5925
Gene Names:RB1
Synonyms:Retinoblastoma-associated protein (p105-Rb) (pRb) (Rb) (pp110)
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-RB (T826) and Total RB PharmaGenie ELISA Kit (SBRS1946) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageHT29 cells were treated with Thymidine-Nocodazole Block. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageHT29 cells were treated with Thymidine and Nocodazole. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

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